Simultaneous Chemo/Enantioseparation and Assay of R-(+)-Rabeprazole and Related Impurities in Pharmaceutical Formulations

Autor:	Jeenet Jayachandran, Manish Gangrade, P. Parthiban, P. Balamurugan, K. Anver Basha
Rok vydání:	2015
Předmět:	Aqueous solution Chromatography Silica gel Organic Chemistry Clinical Biochemistry medicine.disease_cause Biochemistry Analytical Chemistry Volumetric flow rate chemistry.chemical_compound chemistry Impurity Phase (matter) medicine Cellulose Acetonitrile Ultraviolet
Zdroj:	Chromatographia. 78:1367-1375
ISSN:	1612-1112 0009-5893
DOI:	10.1007/s10337-015-2968-x
Popis:	Simultaneous chiral and chemoseparation of R-(+)-rabeprazole and related (enantio)impurities was achieved on a cellulose tris-(3,5-dichlorophenylcarbamate) stationary phase chemically bonded to silica gel (Chiralpak IC). A gradient elution was applied in the reverse-phase separation mode. The mobile phase consisted of a mixture of acetonitrile and aqueous phosphate buffer at pH 7. The other operational parameters were flow rate of 1 mL min−1, column temperature of 35 °C and ultraviolet (UV) detection at 282 nm. Quantification limits for R-(+)-rabeprazole and the related impurities ranged in the interval of 0.02–0.03 %. Linear response intervals of 0.02–0.66 % were obtained with UV detection. Validation of the proposed method was achieved according to current regulations in force. For better understanding of the R-(+)-rabeprazole impurity profile, (+)-EMS/MS and MS/MS detection were also used.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6bac8a96ba8cb4bbbd3d4e1f1c7b4c96 https://doi.org/10.1007/s10337-015-2968-x Zobrazit plný text záznamu Full text from SpringerLink