Simplified Photometric Copper-Soap Method for Rapid Assay of Serum Lipase Activity

Autor:	Wilbert J. Zell, James F. Myrtle
Rok vydání:	1975
Předmět:	Chromatography biology Biochemistry (medical) Clinical Biochemistry chemistry.chemical_element Copper Single tube chemistry Rapid assay biology.protein Serum lipase Lipase Normal range Olive oil
Zdroj:	Clinical Chemistry. 21:1469-1473
ISSN:	1530-8561 0009-9147
Popis:	We modified the method of Yang and Biggs [Clin. Chem. 17, 512 (1971)] for greater speed and simplicity. Serum is incubated with an emulsified olive oil substrate, the fatty acids extracted, the copper soap formed, and phases separated, all in a single tube. An aliquot of the organic phase is removed for color development. One analysis can be completed in 35 min, 20 in less than 2 h. The method is accurate and precise. Results correlate well with the unmodified colorimetric method, and with the classical titrimetric lipase procedure. The reference interval (normal range) is 20 to 180 U/liter.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6b9a3bd116014af5e26fd77c269eddd7 https://doi.org/10.1093/clinchem/21.10.1469 Zobrazit plný text záznamu