Effect of Intravenous Immunoglobulin Treatment on the Th1/Th2 Balance in Women with Recurrent Spontaneous Abortions
| Autor: | Olga Graphou, Loukas H. Margaritis, Ageliki Pantazi, Christos Balafoutas, Virginia Kontopoulou, Athanasia Chioti, Christina Tsekoura, Eleftheria Georgiadou, Marighoula Varla-Leftherioti, S. Koussoulakos |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
biology
business.industry medicine.medical_treatment Immunology Obstetrics and Gynecology Interleukin T helper cell Immunoglobulin E Group B Cytokine medicine.anatomical_structure Reproductive Medicine biology.protein Immunology and Allergy Medicine Interferon gamma Antibody business Interleukin 4 medicine.drug |
| Zdroj: | American Journal of Reproductive Immunology. 49:21-29 |
| ISSN: | 1046-7408 |
| DOI: | 10.1034/j.1600-0897.2003.01169.x |
| Popis: | PROBLEM: The way by which intravenous immunoglobulin (IvIg) acts to prevent immunlogically mediated recurrent spontaneous abortions (RSA) has not been clarified. In the present study, a possible effect of IvIg on the T helper cell (Th1/Th2) balance was investigated in abortions of either alloimmune or autoimmune abnormalities. METHOD OF STUDY: The study included 21 women treated with IvIg before conception because of a history of RSA characterized by alloimmune abnormalities (n = 15) or associated with anti-phospholipid antibodies (APA) (n = 6). Peripheral blood samples, collected before and 5 days after the first IvIg infusion, were stimulated, and Th1 and Th2 cells were detected by flow-cytometric analysis using a combination of monoclonal antibodies against T-cell surface markers and intracellular interferon (IFN)-γ and interleukin (IL)-4. The percentage of IFN-γ-producing (Th1) and IL-4-producing (Th2) cells and the Th1/Th2 ratio were compared between pre- and post-infusion samples. RESULTS: A decrease of Th1 percentage in 66.6% of the cases and a concurrent Th2 percentage increase (47.61%) resulted in a decrease in the Th1/Th2 ratio in most of the cases (76.1%) (p |
| Databáze: | OpenAIRE |
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