Metabolic profiling of icaritin in rats using UHPLC-Q/TOF-MS
| Autor: | Xinchi Feng, Feng Qiu, Xu-Liu Shi, Liwei Chai, Kun Wang, Liqin Ding, Tong Wang, Yang Li |
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| Rok vydání: | 2019 |
| Předmět: |
Pharmacology
Chromatography Uhplc q tof ms Chemistry Metabolite Urine Mass spectrometry 030226 pharmacology & pharmacy 01 natural sciences High-performance liquid chromatography 0104 chemical sciences Hydroxylation 010404 medicinal & biomolecular chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Complementary and alternative medicine In vivo Pharmacology (medical) Glucuronide |
| Zdroj: | Chinese Herbal Medicines. 11:185-191 |
| ISSN: | 1674-6384 |
| Popis: | Objective To identify the in vivo metabolites of icaritin and speculate its metabolic profiling in rats. Methods The plasma, bile, urine, and feces of rats were collected after orally administration of icaritin at a dose of 100 mg/kg and detected by an ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS/MS) in both positive and negative modes. The data of treated and control groups were compared and analyzed with the aid of Metabolynx XS software. Results A total of 25 metabolites were identified in the biosamples, and 14 of them were reported for the first time to our knowledge. Conclusion The main metabolite types of icaritin in rats were glucuronide conjugation, methylation, hydroxylation, reduction, and acetylation. |
| Databáze: | OpenAIRE |
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