Label-free protein detection using a microfluidic Coulter-counter device
| Autor: | Romen Rodriguez-Trujillo, Casper Hyttel Clausen, F. Larsen, Mikkel Dysseholm Mar, Winnie Edith Svendsen, Mohammad Akram Ajine, A. Orzan |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Analyte Chromatography Materials science Microfluidics Metals and Alloys Analytical chemistry Polymer Condensed Matter Physics Buffer (optical fiber) Surfaces Coatings and Films Electronic Optical and Magnetic Materials Suspension (chemistry) chemistry Coulter counter Electrode Materials Chemistry Surface modification Electrical and Electronic Engineering Instrumentation |
| Zdroj: | Sensors and Actuators B: Chemical. 190:922-927 |
| ISSN: | 0925-4005 |
| Popis: | A new method for measuring specific protein concentrations in solutions has been developed. The technique makes use of the Coulter effect for detecting and sizing of micro-scaled objects suspended in a buffer fluid. The method is completely label-free as it is only based on the electrical readout when a suspension of microscopic beads flows over a set of electrodes in a microfluidic device. Since no electrode functionalization is needed the same device can be used in a number of different assays. Using goat-anti-rat IgG functionalized polystyrene beads we have shown proof of principle detecting rat IgG in solution. When the analyte (rat IgG) is present oligomers of beads are formed. The electrical readout of the oligomers is different compared to a zero control sample with no rat IgG. Detection of the protein has been performed in a concentration as small as 14 ng/mL. The dynamic range of the system has been demonstrated to be relatively large, ranging from 1 μg/mL to 14 ng/mL. The microfluidic system is made from polymer and glass and very little volume of sample ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect