A new detection technique for fluoroquinolone-conjugated proteins by high performance liquid chromatography with UV/fluorescence detectors
| Autor: | Bo-Rui Chen, Ching-Wen Shih, Ching-Yu Tu, Yu-Fen Ling, Shao-Wen Hung, Li-Tse Tsou, Shu-Peng Ho, Way-Shyan Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
0303 health sciences Chromatography biology 030309 nutrition & dietetics Danofloxacin Chemistry 010401 analytical chemistry 01 natural sciences Fluorescence High-performance liquid chromatography 0104 chemical sciences 03 medical and health sciences biology.protein medicine Enrofloxacin Ofloxacin Orbifloxacin Bovine serum albumin Food Science medicine.drug Conjugate |
| Zdroj: | Journal of Food and Drug Analysis. 15 |
| ISSN: | 2224-6614 |
| Popis: | This study was aimed to develop a simple, fast, and reliable technique to detect fluoroquinolones (FQs)-conjugated bovine serum albumin (BSA). Four tested FQs, enrofloxacin, ofloxacin, danofloxacin, and orbifloxacin, were conjugated with BSA by following the N-hydroxysuccinimide ester method. The technique was designed according to the different absorption characteristics of the FQs and BSA; FQs can be detected by both UV and fluorescence detectors, but BSA can only be detected by UV. The results demonstrated that the developed method was efficient in detecting FQs-BSA conjugates. In addition, the method not only trace the FQs and BSA conjugation responses but also can be used to estimate the level of FQs-BSA conjugation. Therefore, this technique is a valuable tool for the detection drug-carrier-conjugated antigens, especially for FQs-BSA-conjugates during the production of anti-FQs monoclonal antibodies. |
| Databáze: | OpenAIRE |
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