Supercritical fluid chromatographic determination of tocopherols on an ODS-silica gel column

Autor:	Takashi Yarita, Kouichi Abe, Yasuhiko Takeshita, Akira Nomura
Rok vydání:	1994
Předmět:	Chromatography Silica gel Organic Chemistry food and beverages General Medicine Biochemistry High-performance liquid chromatography Supercritical fluid Capacity factor Analytical Chemistry chemistry.chemical_compound Homologous series chemistry Supercritical fluid chromatography Tocopherol Methanol
Zdroj:	Journal of Chromatography A. 679:329-334
ISSN:	0021-9673
Popis:	A method for the supercritical fluid chromatographic (SFC) determination of tocopherots in vegetable oils was investigated using an ODS-silica gel column with carbon dioxide as the mobile phase. The retention of tocopherols was affected by the density of the mobile phase and the addition of methanol as a modifier. The addition of small concentrations of methanol produced a satisfactory separation of tocopherol homologues, including the positional isomers β- and γ-tocopherol. The results of the determination of tocopherols in vegetable oils by SFC were in satisfactory agreement with those obtained by normal-phase HPLC.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6b7a52e344c9adab8e3663d52ad744d7 https://doi.org/10.1016/0021-9673(94)80575-x Zobrazit plný text záznamu