Structure des acides corynomycoliques de Corynebacterium hofmanii et leur implication biogenetique
| Autor: | J Asselineau, M A Lanéelle, M Welby-Gieusse |
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| Rok vydání: | 1970 |
| Předmět: | |
| Zdroj: | European Journal of Biochemistry. 13:164-167 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1970.tb00913.x |
| Popis: | The three main components of the mixture of corynomycolic acids (α-branched, β-hydroxy acids of the general formula R1—CHOH—CHR2—COOH) isolated from a strain of Corynebacterium hofmanii are: C32H64O3 (corynomycolic acid); C34H66O3 (corynomycolenic acids); C36H68O3 (corynomycoldienic acid). By thin-layer chromatography on silver nitrate-impregnated Silicagel G, we were able to separate the hydroxy acids having 0, 1 or 2 double bonds. The pyrolytic chromatography of their methyl esters indicates that the mono-unsaturated acids form a mixture of C34-acids containing the double bond either in the main chain or in the side chain; the di-unsaturated acid fraction is constituted solely by a C36-acid with a double bond in each fragment arising from pyrolysis. By oxidative cleavage, we find that the position of the double bond is 9, 10 (as in oleic acid). According to the nature of the main chain (aldehyde fragment of pyrolysis) and of the side chains (ester fragment of pyrolysis), some suggestions are made about the selective use of fatty acids in the biosynthesis of corynomycolic acids. |
| Databáze: | OpenAIRE |
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