Rapid and sensitive determination of neomycin and kanamycin in measles, mumps, and rubella vaccine via high‐performance liquid chromatography–tandem mass spectrometry using modified super-paramagnetic Fe3O4 nanospheres

Autor: Alireza Ghasempour, Mahshid Zarrineh, Ali Es-haghi, Hamideh Hamidi
Rok vydání: 2020
Předmět:
Zdroj: Journal of Chromatography A. 1625:461343
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2020.461343
Popis: A simple magnetic dispersive solid-phase extraction (MDSPE) methodology based on mesoporous Fe3O4@ succinic acid nanospheres and high‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS) has been developed to determine kanamycin (KNM) and neomycin (NEO) contents in Measles, Mumps, and Rubella (MMR) vaccine products. The monodispersed mesoporous Fe3O4 nanospheres with self-assembled carboxyl terminated shell have been prepared via a simple solvothermal method. These as-synthesized mesoporous Fe3O4 nanospheres showed a high magnetic saturation value (Ms = 46 emu g−1) and large specific surface area (111.12 m2 g−1) which made them potential candidates as sorbents in magnetic solid-phase extraction. The adsorption experimental data fitted well with the Freundlich-Langmuir isotherm and followed a pseudo-second-order kinetic model. Moreover influential parameters on extraction efficiency were investigated and optimized. Under optimal conditions, the limits of detection for KNM and NEO were 1.0 and 0.1 ng mL−1, respectively. Recovery assessments using real samples exhibited recoveries in the range of 96.0 ± 4.3 to 101.5 ± 7.1 %, with relative standard deviations of
Databáze: OpenAIRE
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