The effect of CD34 + cell telomere length and hTERT expression on the outcome of autologous CD34 + cell transplantation in patients with chronic heart failure

Autor: Metka Krasna, Srdjan Novaković, Mojca Jez, Maja Pohar Perme, Elvira Malicev, Bojan Vrtovec, Jasmina-Ziva Rozman, Primoz Rozman
Rok vydání: 2017
Předmět:
Zdroj: Mechanisms of Ageing and Development. 166:42-47
ISSN: 0047-6374
DOI: 10.1016/j.mad.2017.06.001
Popis: Age-related telomere attrition in stem/progenitor cells may diminish their functional capacity and thereby impair the outcome of cell-based therapies. The aim of the present study was to investigate the effect of CD34+ cell telomere length and hTERT expression on the clinical outcome of autologous CD34+ cell transplantation. We studied 43 patients with cardiomyopathy. Their peripheral blood CD34+ cells were mobilized with granulocyte colony-stimulating factor, enriched by immunoselection and delivered transendocardially. Relative telomere length and expression levels of hTERT were measured using a real-time PCR assay. Immunoselected CD34+ cells had longer telomere length compared to leukocytes in leukapheresis products (p=0.001). In multivariate analysis, CD34+ cell telomere length was not associated with the clinical outcome (b=3.306, p=0.540). While hTERT expression was undetectable in all leukapheresis products, 94.4% of the CD34+ enriched cell products expressed hTERT. Higher CD34+hTERT expression was associated with a better clinical outcome on univariate analysis (b=87.911, p=0.047). Our findings demonstrate that CD34+ cell telomere length may not influence the clinical outcome in cardiomyopathy patients treated with autologous CD34+ cell transplantation. Larger studies are needed to validate the impact of the CD34+hTERT expression on the clinical outcome of autologous CD34+ cell transplantation.
Databáze: OpenAIRE
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