| Autor: |
Olaf Wiest, Nicolas Saettel |
| Rok vydání: |
2006 |
| Předmět: |
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| Zdroj: |
Tetrahedron. 62:6490-6500 |
| ISSN: |
0040-4020 |
| DOI: |
10.1016/j.tet.2006.03.059 |
| Popis: |
The electron transfer catalyzed cycloreversion of cyclobutane pyrimidine dimers is the key step in repair of light-induced DNA lesions catalyzed by the enzyme CPD photolyase. The formation of the CPD radical anion was found to be strongly solvent dependent due to a specific hydrogen bond that stabilizes the valence bound state over the dipole bound state of the additional electron. The effect of solvation on the vertical and adiabatic electron affinity of uracil and uracil dimers as well as on the mechanism of the cycloreversion of the uracil dimer radical anion is explored for three model systems that include explicit solvent molecules at the B3LYP/6-311++G ∗∗ /B3LYP/6-31+G ∗ level of theory. The second solvation shell is described using the implicit C-PCM solvation model. These calculations indicate an effectively barrierless mechanism. These results are in agreement with the available experimental data for the reaction energies and isotope effects. It is also shown that a single hydrogen bond donor is a sufficient minimal model for the first solvation shell by adequately describing the stabilization of the valence bound state of the radical anion through hydrogen bonding. The relationship of these model systems with the enzymatic reaction catalyzed by DNA photolyase is also discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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