A Genosensor for Point Mutation Detection of P53 Gene PCR Product Using Magnetic Particles

Autor:	Ezat Hamidi-Asl, Simin Sharifi, Marco Mascini, Mohammad Saeid Hejazi, S. M. Golabi, Jahan-Bakhsh Raoof, Ilaria Palchetti
Rok vydání:	2015
Předmět:	Streptavidin chemistry.chemical_compound Chemistry Biotinylation Point mutation Mutant Electrochemistry Gene Biosensor Molecular biology DNA sequencing DNA Analytical Chemistry
Zdroj:	Electroanalysis. 27:1378-1386
ISSN:	1040-0397
DOI:	10.1002/elan.201400660
Popis:	The development of an electrochemical genosensor involving DNA biotinylated capture probe immobilized on streptavidin coated paramagnetic beads and microfluidic based platform for the detection of P53 gene PCR product is reported. The novelty of this work is the combination of a sensitive electrochemical platform and a proper microfluidic system with a simple and effective enzyme signal amplification technology, ELISA, for detection of target DNA sequence and single nucleotide mutation in p53 tumor suppressor gene sequence. The biosensor has been applied to detect the PCR amplified samples and the results shows that it can discriminate successfully perfect matched DNA from mutant form.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6b15eb472f35f0190d536e52ce8234e4 https://doi.org/10.1002/elan.201400660 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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