ASSESSMENT OF GENETIC VARIABILITY OF BAMBARA GROUNDNUT (VIGNA SUBTERRANEAN (L.) VERDC.) ACCESSIONS USING MORPHOLOGICAL TRAITS AND MOLECULAR MARKERS
| Autor: | S.M. Basu, O. Molosiwa, S. Azam-Ali, S. Mayes, F. Stadler |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Acta Horticulturae. :779-790 |
| ISSN: | 2406-6168 0567-7572 |
| Popis: | The efficiency of breeding can be improved by employing a number of genetic diversity measures of the crop germplasm. Genetic diversity and relatedness of 24 Bambara groundnut accessions (Vigna subterranean L. Verdc.) were estimated using 36 morphological characters, 201 Diversity Array Technology (DArT) and 65 simple sequence repeat (SSR) markers to assess the relationships between the material and also between the three marker types. These accessions were selected to represent the diversity within cultivated Bambara groundnut germplasm. A broader group of 119 Bambara groundnut accessions (87 from International Institute of Tropical Agriculture and 37 from University of Nottingham stocks; three seeds per accession) were planted in an unheated glasshouse at University of Nottingham in May, 2008. Data on the variation for morphological and agronomic traits were recorded following the IPGRI descriptors (IITA, BAMNET (2000). Samples were also analysed with microsatellite markers which have been characterised in this project. In addition, DNA extracted from the 119 accessions was sent for DArT analysis. This study has demonstrated the utility of the three marker types for genetic diversity and relatedness analysis. The genetic distance (GD) among the landraces and the first two axes for the principal component analysis (PCA) were obtained. The highest average genetic distance (GD) was obtained using morphological marker (0.55) followed by DArT (0.43) and SSR marker (0.25). The estimated values of the genetic relationship between all the markers were significantly correlated at (P |
| Databáze: | OpenAIRE |
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