Experimental concerns in tracer kinetic investigations of apolipoprotein metabolism
| Autor: | Waldo R. Fisher, Bruce W. Patterson |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Statistics and Probability
Plasma lipoprotein Tracer kinetic General Immunology and Microbiology Apolipoprotein B biology Chemistry Applied Mathematics General Medicine Metabolism Computational biology General Biochemistry Genetics and Molecular Biology Biochemistry Modelling methods Modeling and Simulation Plasma lipids biology.protein lipids (amino acids peptides and proteins) Lipoprotein metabolism General Agricultural and Biological Sciences Vldl metabolism |
| Zdroj: | Mathematical Biosciences. 72:387-405 |
| ISSN: | 0025-5564 |
| DOI: | 10.1016/0025-5564(84)90120-2 |
| Popis: | The complexity of the metabolism of the plasma lipoproteins makes it impossible to integrate the details of the reactions of specific apolipoproteins and their associated lipids without the use of computerized modeling methods. Because apolipoproteins impart specificity in the transport and chemical processing of plasma lipids, they have been the focus of many in vivo kinetic tracer investigations. The analysis of such kinetic data by modeling techniques has provided important advances in understanding lipoprotein metabolism. An example is the Delipidation Chain, an hypothesis explaining VLDL metabolism in terms of a sequential delipidation process. As a consequence of the advance in knowledge of apolipoprotein structure and metabolism, coupled with progress in computerized modeling of large systems, it has become important to refine the design of in vivo tracer kinetic investigations of the apolipoproteins. Considerations of particular importance include the selection of apolipoprotein tracers which can be shown to undergo the same reactions as the apolipoproteins whose metabolism they trace. If the physical and chemical processes which convert apolipoproteins from one metabolic pool to another are to be analyzed correctly, it is necessary to describe precisely and to measure accurately these pools. Current methods for delineating metabolic pools of apolipoproteins in vivo need to be refined. When accomplished, this will provide new opportunities to investigate the metabolic pathways of the apolipoproteins and their associated lipids. A very important challenge is to design experiments which will differentiate transfer processes, which result in net transport of a reactant, from exchange processes, whereby a tracer and a tracee are exchanged between pools without a net transport event occuring. Since both types of processes occur readily with apolipoproteins, it is important to develop methods to examine them separately. Computerized kinetic modeling provides a means for describing and understanding the complexities of lipoprotein metabolism. A major challenge is for the experimentalist to acquire data which accurately reflect the physiological processes involved in lipoprotein metabolism. |
| Databáze: | OpenAIRE |
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