| Popis: |
The following staining procedure is recommended for use in the Brucella opsonocytophagic test in order to avoid confusing results obtained with stains of the Hasting or Wright type: Fix spreads for 5 minutes or longer in absolute methyl alcohol. Stain for 10 to 30 minutes in a solution of the following: 0.5 g. NaCl, 0.5 g. phenol, 0.5 g. methylene blue, 0.02 g. Na2HPO4+12H2O, 50 cc. distilled water, 50 cc. methyl alcohol. Wash slides gently in water. Air dry. By this procedure, the bacteria and the nuclei of the leucocytes appear deep blue. The cytoplasm of the leucocytes appears faintly green with the cell outline distinctly visible. Cytoplasmic granules do not stain. |
