Abstract A25: Molecular characterization of cyclin-dependent kinase 1 pathway in newly established epithelial ovarian cancer cell lines

Autor: Ha-Yeon Shin, Jae Hoon Kim, Sol Kim, Eun Ju Lee, Woo Kyeom Yang, Hanbyoul Cho, Assel Sabrgaliyeva
Rok vydání: 2015
Předmět:
Zdroj: Molecular Cancer Therapeutics. 14:A25-A25
ISSN: 1538-8514
1535-7163
DOI: 10.1158/1538-8514.tumang15-a25
Popis: Background: Epithelial ovarian cancer (EOC) is a heterogeneous gynecologic tumor. To better understand the biological pathways involved in the development of EOC, we performed mRNA expression profiling on newly established EOC cell lines and studied the common characteristics in EOC cells. Methods: Five newly established EOC cell lines were characterized by transcript profiling. Microarray analyses were validated using quantitative real-time PCR (RT-PCT), immunoblotting, and GEO dataset. Subsequently, we investigated the possible functions of S100A14 in EOC by small interfering RNA (siRNA) approaches. Results: A total of 239 genes represented by 24,957 probes were identified from microarray analysis, of which 98 and 141 genes are up- (> 2 fold, p < 0.05) or down-regulated (< 3 fold, p < 0.05), respectively, in EOC cell lines compare to human ovarian surface epithelial cells. Interestingly, our data showed upregulation of 11 genes which are associated with the CDK1 pathway. In RT-PCR and immunoblotting, Cdk1 and cyclin B1 expression was significantly increased in EOC cells and tissues compared to benign or normal ovarian surface epitheliums. Cdk1 knockdown by siCdk1 or Cdk1 inhibitor, RO-3306, inhibited cell growth and arrested the cell cycle at G2/M phase with increased pro-apoptotic signals and decreased anti-apoptotic signals. Conclusions: These results suggest that elevated expression of Cdk1/cyclinB1 is important to EOC development and progression, providing new insight into the biology of EOC. Citation Format: Hanbyoul Cho, Assel Sabrgaliyeva, Woo Kyeom Yang, Sol Kim, Ha-Yeon Shin, Eun Ju Lee, Jae-hoon Kim. Molecular characterization of cyclin-dependent kinase 1 pathway in newly established epithelial ovarian cancer cell lines. [abstract]. In: Proceedings of the AACR Special Conference: Tumor Angiogenesis and Vascular Normalization: Bench to Bedside to Biomarkers; Mar 5-8, 2015; Orlando, FL. Philadelphia (PA): AACR; Mol Cancer Ther 2015;14(12 Suppl):Abstract nr A25.
Databáze: OpenAIRE
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