Production, freezing and transfer of bovine IVF embryos and subsequent calving results

Autor: S.A. Trimmer, B. Neely, L.S. Shuey, A.D. McCauley, W.B. Henderson, Z.Q. Jin, P.J. Hurtgen, J.E. Stokes, J.F. Hasler, S.A. Mower
Rok vydání: 1995
Předmět:
Zdroj: Theriogenology. 43:141-152
ISSN: 0093-691X
DOI: 10.1016/0093-691x(94)00020-u
Popis: Ultrasound-guided oocyte aspirations were performed repeatedly, on a weekly basis, on 155 different cows. An average of 4.9 oocytes with 4.1 classified as usable were collected. Following in vitro maturation (IVM), fertilization (IVF) and culture (IVC), a few Day 7 morulae, and all Day 7 and most Day 8 blastocysts were either transferred or frozen. The transfer of 2268 fresh IVF embryos resulted in 1220 pregnancies (53 8%). Day 7 embryos resulted in a higher (56%) pregnancy rate than did Day 8 (43%) or Day 9 (41%). In addition, the transfer of embryos classified as grade 1 on either Day 7 or 8 resulted in a higher pregnancy rate than did grade 2 embryos. Although there was no difference in pregnancy rate, embryos resulting from co-culture with Buffalo Rat Liver Cells n Menezo's B2 medium developed faster than embryos co-cultured with TCM 199 culture medium. Pregnancy rate following transfer of IVF blastocysts frozen on Day 7 was 42% and dropped to 20% for blastocysts frozen on Day 8. Pregnancy rate was not influenced when synchrony between the IVF embryo and recipient was within ± 36 h. The sex ratio of the resulting calves did not deviate from normal but there was a higher than normal incidence of early abortions and dystocias. There appeared to be some increase in calf birth weights.
Databáze: OpenAIRE
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