Identification, characterization and leucocyte expression of Siglec-10, a novel human sialic acid-binding receptor
| Autor: | James MUNDAY, Sheena KERR, Jian NI, Ann L. CORNISH, Jiquan Q. ZHANG, Gavin NICOLL, Helen FLOYD, Marie-Genevieve MATTEI, Paul MOORE, Ding LIU, Paul R. CROCKER |
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| Rok vydání: | 2001 |
| Předmět: | |
| Zdroj: | Biochemical Journal. 355:489-497 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj3550489 |
| Popis: | Here we characterize Siglec-10 as a new member of the Siglec family of sialic acid-binding Ig-like lectins. A full-length cDNA was isolated from a human spleen library and the corresponding gene identified. Siglec-10 is predicted to contain five extracellular Ig-like domains and a cytoplasmic tail containing three putative tyrosine-based signalling motifs. Siglec-10 exhibited a high degree of sequence similarity to CD33-related Siglecs and mapped to the same region, on chromosome 19q13.3. The expressed protein was able to mediate sialic acid-dependent binding to human erythrocytes and soluble sialoglycoconjugates. Using specific antibodies, Siglec-10 was detected on subsets of human leucocytes including eosinophils, monocytes and a minor population of natural killer-like cells. The molecular properties and expression pattern suggest that Siglec-10 may function as an inhibitory receptor within the innate immune system. |
| Databáze: | OpenAIRE |
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