Evaluation of rapid test kits for quantification of HT-2 and T-2 toxins in naturally contaminated oats

Autor:	B. Holen, S.S. Klemsdal, O. Elen, Guro Brodal, H.U. Aamot, Ingerd Skow Hofgaard
Rok vydání:	2013
Předmět:	Fusarium biology Toxin business.industry Public Health Environmental and Occupational Health Contamination Toxicology medicine.disease_cause biology.organism_classification Biotechnology chemistry.chemical_compound chemistry medicine Food science business Mycotoxin Food Science
Zdroj:	World Mycotoxin Journal. 6:31-41
ISSN:	1875-0796 1875-0710
Popis:	The aim of this study was to evaluate the performance and usefulness of three rapid test kits for analysis of HT-2 and T-2 toxins (HT-2 and T-2), two of the most potent trichothecenes commonly found in European oats. Concentrations of these two toxins combined (HT-2+T-2) were analysed in naturally contaminated oat samples (n=68) using the following test kits: Ridascreen® FAST T-2 Toxin (‘Fast ELISA’), DRAFT Ridascreen® HT-2/T-2 (‘Standard ELISA’, not commercially available), and the lateral flow device ROSA® HT-2-T-2 (‘Rosa LFD’). Mycotoxin analysis by LC-MS/MS was used as a reference method. Rosa LFD offered the best reliability, achieving detection that was stable across toxin levels, whereas detection by both ELISA kits differed significantly among toxin levels (P2=0.94), and the corresponding R2 values for Fast and Standard ELISA were 0.61 and 0.83, respectively. Rosa LFD was well suited for on-site detection. Standard ELISA allows simultaneous testing of several samples that are useful for centralised laboratories.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6ac876ca4cc32c409820def4029accb0 https://doi.org/10.3920/wmj2012.1496 Zobrazit plný text záznamu