Integrated culture platform based on a human platelet lysate supplement for the isolation and scalable manufacturing of umbilical cord matrix-derived mesenchymal stem/stromal cells
| Autor: | Shi-Hwei Liu, Francisco Moreira, Cláudia Lobato da Silva, Chen-Peng Ku, Ana Fernandes-Platzgummer, Joaquim M. S. Cabral, William Milligan, António M. de Soure, Carla Lilaia, Yi-Feng Huang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Stromal cell Mesenchymal stem cell Biomedical Engineering Medicine (miscellaneous) Microcarrier Biology Regenerative medicine Cord lining Cell biology Biomaterials 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Stem Cell Isolation 030220 oncology & carcinogenesis Cell adhesion Biomedical engineering Explant culture |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine. 11:1630-1640 |
| ISSN: | 1932-6254 |
| DOI: | 10.1002/term.2200 |
| Popis: | Umbilical cord matrix (UCM)-derived mesenchymal stem/stromal cells (MSCs) are promising therapeutic candidates for regenerative medicine settings. UCM MSCs have advantages over adult cells as these can be obtained through a non-invasive harvesting procedure and display a higher proliferative capacity. However, the high cell doses required in the clinical setting make large-scale manufacturing of UCM MSCs mandatory. A commercially available human platelet lysate-based culture supplement (UltraGROTM, AventaCell BioMedical) (5%(v/v)) was tested to effectively isolate UCM MSCs and to expand these cells under (1) static conditions, using planar culture systems and (2) stirred culture using plastic microcarriers in a spinner flask. The MSC-like cells were isolated from UCM explant cultures after 11 ± 2 days. After five passages in static culture, UCM MSCs retained their immunophenotype and multilineage differentiation potential. The UCM MSCs cultured under static conditions using UltraGROTM-supplemented medium expanded more rapidly compared with UCM MSCs expanded using a previously established protocol. Importantly, UCM MSCs were successfully expanded under dynamic conditions on plastic microcarriers using UltraGROTM-supplemented medium in spinner flasks. Upon an initial 54% cell adhesion to the beads, UCM MSCs expanded by >13-fold after 5–6 days, maintaining their immunophenotype and multilineage differentiation ability. The present paper reports the establishment of an easily scalable integrated culture platform based on a human platelet lysate supplement for the effective isolation and expansion of UCM MSCs in a xenogeneic-free microcarrier-based system. This platform represents an important advance in obtaining safer and clinically meaningful MSC numbers for clinical translation. Copyright © 2016 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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