Hyphenated LC-ABTS·+ and LC-DAD-HRMS for simultaneous analysis and identification of antioxidant compounds in Astragalus emarginatus Labill. extracts
| Autor: | Saïd Ennahar, Rammal Hassan, Chamandy Abir, Zhao Minjie |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
chemistry.chemical_classification
Chromatography ABTS Antioxidant Chemistry medicine.medical_treatment Trolox equivalent antioxidant capacity Pharmaceutical Science Glycoside Pharmacy Analytical Chemistry chemistry.chemical_compound Drug Discovery Apigenin Electrochemistry Caffeic acid medicine Quercetin Spectroscopy Isorhamnetin |
| Zdroj: | Journal of Pharmaceutical Analysis. 12:253-262 |
| ISSN: | 2095-1779 |
| Popis: | The compounds in leaf and stem extracts of Astragalus emarginatus Labill., a plant species used in traditional Lebanese medicine, were investigated for antioxidant properties. First, the activity of various extracts was assessed using the Trolox equivalent antioxidant capacity, oxygen radical absorption capacity, and 2,2-diphenyl-1-picryl-hydrazyl-hydrate assays. The extract obtained using 30% ethanol showed the greatest activity. The antioxidant compounds in this extract were screened using a hyphenated high-performance liquid chromatography-2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) radical system before being separated by ultra-high-performance liquid chromatography and identified using high-resolution mass spectrometry and ultra-violet-visible diode array detection. Approximately 40 compounds were identified. Hydroxycinnamates (caffeic, ferulic, and p-coumaric acid derivatives) and flavonoids (quercetin, luteolin, apigenin, and isorhamnetin derivatives) were the two main categories of the identified compounds. The active compounds were identified as caffeic acid derivatives and quercetin glycosides. In addition, the catechol moiety was shown to be key to antioxidant activity. This study showed that A. emarginatus Labill. is a source of natural antioxidants, which may explain its medicinal use. |
| Databáze: | OpenAIRE |
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