Taxol production in suspension cultures of Taxus baccata

Autor:	Michael L. Shuler, Luis J. Pestchanker, Thomas J. Hirasuna, Venkatesh Srinivasan
Rok vydání:	1996
Předmět:	Taxane biology organic chemicals Plant tissue culture Picloram Fructose Horticulture biology.organism_classification chemistry.chemical_compound Tissue culture chemistry Biochemistry Taxus Extracellular Geraniol
Zdroj:	Plant Cell, Tissue and Organ Culture. 44:95-102
ISSN:	1573-5044 0167-6857
DOI:	10.1007/bf00048185
Popis:	The response of Taxus baccata (PC2) to basic manipulations of culture conditions is described. Suspension cultures of Taxus baccata (PC2) were maintained at 25°C on a modified B5 medium with two-week transfers. Under these conditions, no taxol® is formed. However, if the cells are left in the same medium for 7 or more additional days, taxol is produced and released (ca. 90%) into the extracellular medium. Levels as high as 13 mg 1−1 extracellular taxol were achieved in shake flask cultures and taxol was the primary taxane formed representing between 50 and 80% of total taxane in the medium. The cells are sensitive to changes in culture conditions and cultures cycle through periods of high (13 mg 1−1) and low (
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6a63baf25d1f8384d8c51cb5035cae41 https://doi.org/10.1007/bf00048185 Zobrazit plný text záznamu Full text from SpringerLink