Effect of a Combination of Photodynamic Therapy and Chitosan on Streptococcus mutans (An In Vitro Study)
| Autor: | Hosna Ebrahimi Zadeh, Armin Mirfasihi, Beheshteh Malek Afzali, Maziar Mir, Khashayar Sanjari |
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| Rok vydání: | 2020 |
| Předmět: |
Urology
medicine.medical_treatment Positive control Photodynamic therapy Dermatology Chitosan 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine In vitro study Dentistry (miscellaneous) Orthopedics and Sports Medicine Chromatography biology business.industry 030206 dentistry Bactericidal effect biology.organism_classification Streptococcus mutans McFarland standards chemistry Nephrology Surgery Aseptic processing business |
| Zdroj: | Journal of Lasers in Medical Sciences. 11:405-410 |
| ISSN: | 2228-6721 2008-9783 |
| DOI: | 10.34172/jlms.2020.64 |
| Popis: | Introduction: This study aimed to assess the effect of photodynamic therapy (PDT) and chitosan separately and in combination on Streptococcus mutans. Methods: This in vitro experimental study evaluated 216 microbial samples in 6 groups. First, 5 µL of 0.5 McFarland standard suspension of S. mutans was added to each well of an ELISA microplate; 100 µL of Mueller Hinton broth was also added to each well; 180 wells contained S. mutans suspension while 36 wells were devoid of bacteria. Group 1 served as the negative control and had no bacteria. Group 2 served as the positive control and S. mutans in the positive control wells did not undergo any intervention. In groups 3 and 4, PDT with a 50 mW low-level laser was performed for 30 and 40 seconds respectively. In group 5, 3 mg/mL of chitosan (100 µL) was used. In group 6, 3 mg/mL (100 µL) of chitosan was used in combination with PDT (50 mW laser for 30 seconds). The laser was irradiated under aseptic conditions at a 660 nm wavelength with 50 mW power. Data were analyzed using one-way ANOVA and Tukey's test. Results: PDT combined with chitosan showed maximum bactericidal effect followed by PDT for 40 seconds and chitosan groups (P < 0.05). PDT for 30 seconds showed a minimum bactericidal effect (P < 0.05). All pairwise comparisons revealed significant differences (P < 0.001). Conclusion: Chitosan and PDT alone can be used to decrease the S. mutans count. However, their combined use has a greater bactericidal effect on S. mutans . |
| Databáze: | OpenAIRE |
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