Hydrogen uptake by Robinia pseudoacacia nodules

Autor:	Dietrich Werner, Harold J. Evans, Mechthild Röhm, Wolfgang R. Streit
Rok vydání:	1993
Předmět:	Hydrogenase Rhizobiaceae Ecology biology Physiology Robinia Structural gene food and beverages Nitrogenase Forestry Plant Science biology.organism_classification Biochemistry Nitrogen fixation Rhizobium Bacteria
Zdroj:	Trees. 8
ISSN:	1432-2285 0931-1890
DOI:	10.1007/bf00197687
Popis:	Hydrogen uptake is thought to increase the efficiency of nitrogen fixation by recycling H2 produced by nitrogenase that would otherwise be lost by diffusion. Here we demonstrate the capacity of eight Rhizobium strains to take up molecular hydrogen. Uptake by nodule homogenates from Robinia pseudoacacia was measured amperometrically under nitrogenase repression. Markedly lower activities were found than in soybean nodules. In addition hydrogenase activity was detected by the ability of bacteroids to reduce methylene blue in the presence of hydrogen. It was demonstrated that hydrogenase structural genes are present in the black locust symbiont, Rhizobium sp. strain R1, using hybridization with a plasmid, which contained hydrogenase genes from R. leguminosarum bv. viceae.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6a51ed693a9cb5ff373ed886f24e87d2 https://doi.org/10.1007/bf00197687 Zobrazit plný text záznamu Full text from SpringerLink