Using High-Performance Counter-Current Chromatography Combined with Preparative High Performance Liquid Chromatogramphy for the Separation of Bioactive Compounds from the Water Extract ofGentiana macrophyllaPall
| Autor: | Aihuan Peng, Jinliang Yang, Haoyu Ye, Huijun Lai, Shijie Zhong, Minghai Tang, Shucai Li, Shicao He, Lijuan Chen, Jian Zhao, Wenshuang Wu, Jie Shi |
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| Rok vydání: | 2012 |
| Předmět: |
Solvent system
Chromatography biology Isoorientin Process Chemistry and Technology General Chemical Engineering Filtration and Separation General Chemistry biology.organism_classification High-performance liquid chromatography Gentiana macrophylla chemistry.chemical_compound Loganic acid Countercurrent chromatography Uv spectra chemistry Macrophylloside |
| Zdroj: | Separation Science and Technology. 47:762-768 |
| ISSN: | 1520-5754 0149-6395 |
| DOI: | 10.1080/01496395.2011.621920 |
| Popis: | In this paper, a combined high performance counter-current chromatography (HPCCC) and preparative high-performance liquid chromatography (HPLC) method was employed for rapid separation and enrichment of bioactive constituents from a water extract of Gentiana macrophylla Pall. With a two phase solvent system composed of ethyl acetate-n-butanol-water-acetic acid (2: 3: 5: 0.6, v/v), the water extract of G. macrophylla Pall was fractionated into six fractions with three targets isolated and four others highly concentrated, which were then further purified by preparative-HPLC. As a result, 37 mg deglucoserrulatoside, 22.4 mg loganic acid, 3.9 mg isoorientin, 22.4 mg swertiamarin, 52.3 mg gentiopicroside, 27.5 mg sweroside, and 7.9 mg macrophylloside D with the purity of 95.3%, 90.2%, 98%, 98%, 99.2%, 98.8%, and 98.4%, respectively, were isolated from the water extract of Gentiana macrophylla Pall. The structures were confirmed by UV spectra, MS, as well as NMR measurements. |
| Databáze: | OpenAIRE |
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