ChemInform Abstract: Identification of a Metagenome-Derived Esterase with High Enantioselectivity in the Kinetic Resolution of Arylaliphatic Tertiary Alcohols

Autor: David Weiner, Uwe T. Bornscheuer, Sajja Hari Krishna, Robert Kourist, Flash Bartnek, Jesal S. Patel, Tim Hitchman
Rok vydání: 2008
Předmět:
Zdroj: ChemInform. 39
ISSN: 1522-2667
0931-7597
DOI: 10.1002/chin.200805211
Popis: 35 metagenome-derived esterases bearing a GGG(A)X motif were screened for activity and enantioselectivity in the hydrolysis of a range of tertiary alcohol acetates. Most of the active esterases showed little or no enantioselectivity in the hydrolysis of the terpinyl acetate, linalyl acetate and 3-methylpent-1-yn-3-yl acetate. However, one esterase showed excellent enantioselectivity (E > 100) in the kinetic resolution of 1,1,1-trifluoro-2-phenylbut-3-yn-2-yl acetate as confirmed by a preparative scale reaction.
Databáze: OpenAIRE