ChemInform Abstract: Identification of a Metagenome-Derived Esterase with High Enantioselectivity in the Kinetic Resolution of Arylaliphatic Tertiary Alcohols
Autor: | David Weiner, Uwe T. Bornscheuer, Sajja Hari Krishna, Robert Kourist, Flash Bartnek, Jesal S. Patel, Tim Hitchman |
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Rok vydání: | 2008 |
Předmět: | |
Zdroj: | ChemInform. 39 |
ISSN: | 1522-2667 0931-7597 |
DOI: | 10.1002/chin.200805211 |
Popis: | 35 metagenome-derived esterases bearing a GGG(A)X motif were screened for activity and enantioselectivity in the hydrolysis of a range of tertiary alcohol acetates. Most of the active esterases showed little or no enantioselectivity in the hydrolysis of the terpinyl acetate, linalyl acetate and 3-methylpent-1-yn-3-yl acetate. However, one esterase showed excellent enantioselectivity (E > 100) in the kinetic resolution of 1,1,1-trifluoro-2-phenylbut-3-yn-2-yl acetate as confirmed by a preparative scale reaction. |
Databáze: | OpenAIRE |
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