Paper-Based Neuraminidase Assay Sensor for Detection of Influenza Viruses
| Autor: | Chang-Soo Lee, Yun-Gon Kim, Cheol-Hwan Hwang, Seong-Geun Jeong, Han-Kyu Park |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Detection limit biology General Chemical Engineering Substrate (chemistry) 02 engineering and technology 021001 nanoscience & nanotechnology humanities Enzyme assay 03 medical and health sciences 030104 developmental biology Enzyme chemistry Biochemistry biology.protein 0210 nano-technology Colorimetric analysis Neuraminidase |
| Zdroj: | Korean Chemical Engineering Research. 54:380-386 |
| ISSN: | 0304-128X |
| DOI: | 10.9713/kcer.2016.54.3.380 |
| Popis: | In this study, we described a paper-based neuraminidase assay sensor (PNAS) which can be applied to detect the infection by influenza viruses. The PNAS was designed and manufactured to quantitatively identify the levels of neuraminidase in the sample, which is based on colorimetric analysis using the X-Neu5Ac substrate. The limit of detection of the PNAS was determined as 0.004 U/mL of neuraminidase. According to the amount of neuraminidase in human serum, the PNAS could monitor the enzyme activity with a good linearity (R 2 > 0.99). In addition, the initial per- formance of the PNAS has been maintained up to 70 days in the 4 o C. Finally, we demonstrated whether the Michaelis- Menten kinetics is applied to the PNAS, which can show the reliability of the enzyme reactions. The kinetic studies indi- cated that the PNAS provides the good condition for enzyme reactions (Km=8.327×10 �3 M), but they were performed on paper chip nonetheless. The paper-based neuraminidase assay sensor may be useful in a wide range of rapid and safe detection of influenza virus. |
| Databáze: | OpenAIRE |
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