Radioiodination and biodistribution of the monoclonalantibody TU-20 and its SCFV fragment

Autor:	H. Kubaštová, V. Kleinová, M. Fišer, Karel Kranda, D. Seifert
Rok vydání:	2006
Předmět:	Gel electrophoresis Biodistribution Chemistry medicine.drug_class General Physics and Astronomy Class iii respiratory system Monoclonal antibody Biochemistry In vivo biodistribution In vivo medicine Neuronal cytoskeleton Immunohistochemistry
Zdroj:	Czechoslovak Journal of Physics. 56:D683-D688
ISSN:	1572-9486 0011-4626
DOI:	10.1007/s10582-006-0565-9
Popis:	The ability of the monoclonal antibody TU-20 and its scFv fragment to specifically bind to the C-end of the class III beta-tubulin makes these preparations useful as potential diagnostics for in vivo determination of neurodegenerative diseases that entail degradation of neuronal cytoskeleton. To examine this hypothesis, TU-20 and its scFv were labelled with 125I and their properties were extensively investigated. TU-20 and its scFv were labelled via chloramine-T with the yield 90–95% and 64–78%, respectively. Their quality control, performed by an ELISA and gel electrophoresis, determined adequate properties for further studies. The in vitro experiment, involving autoradiography and immunohistochemistry of mice’ brain slices, enabled confirmation of preserved immunospecificity of the radiolabelled substances. Finally, the in vivo biodistribution proved differences in elimination of either TU-20, scFv TU-20, or iodide from the mice.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6a3e661628e82883e74d1d51e2f04ff9 https://doi.org/10.1007/s10582-006-0565-9 Zobrazit plný text záznamu Full text from SpringerLink