Direct non-invasive observation of metabolism in living cells by 13C nuclear magnetic resonance spectroscopy

Autor:	John Y. Lee, Philip J. Sidebottom, Gerardo Burton, Kozo Shishido, Paul E. Fagerness, A. Ian Scott, Robert Baxter, J. Martyn Gunn
Rok vydání:	1980
Předmět:	Nuclear magnetic resonance Chemistry Organic Chemistry Non invasive General Chemistry Nuclear magnetic resonance spectroscopy Metabolism Carbon-13 NMR Catalysis
Zdroj:	Canadian Journal of Chemistry. 58:1839-1846
ISSN:	1480-3291 0008-4042
DOI:	10.1139/v80-289
Popis:	Applications of 13C nmr in following the metabolism and fate of 13C enriched substrates in whole cells are described. Studies on the elaboration of coproporphyrinogens I (2a) and III (2b) from [5-13C]-δ-aminolevulinic acid (1) and [11-13C-porphobilinogen (PBG, 3), in Rhodopseudomonasspheroides and Propionibacteriumshermanii, respectively, the randomization of 13C from [3-13C]-propionate by P. shermanii, the biosynthesis of citrate, gentisalcohol (4), and patulin (5) from [2-13C]-acetate by Penicilliumurticae, and the anaerobic metabolism of [1-13C]-glucose in rabbit erythrocytes are reported.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6a3bb7c0c36712df3c07a841cd9adecb https://doi.org/10.1139/v80-289 Zobrazit plný text záznamu