Sheep flock infections with Mycoplasma ovipneumoniae involve multiple strains

Autor:	Maria E. Harvey, Daniel G. Morrical, Ricardo F. Rosenbusch
Rok vydání:	2007
Předmět:	Genetics Gel electrophoresis animal diseases Biology biology.organism_classification law.invention Sheep flock Microbiology Food Animals Mycoplasma ovipneumoniae law Animal Science and Zoology Amplified fragment length polymorphism Flock Primer (molecular biology) Polymerase chain reaction
Zdroj:	Small Ruminant Research. 73:287-290
ISSN:	0921-4488
Popis:	Infections with Mycoplasma ovipneumoniae have been characterized as occurring with multiple strains within a flock of sheep. In this study clonal isolates of M. ovipneumoniae recovered from 35 lambs from five flocks in central Iowa were analyzed. Using a single primer arbitrarily primed polymerase chain reaction (AP-PCR), and amplified-fragment length polymorphism (AFLP) techniques, a genomic fingerprint was created for each M. ovipneumoniae clonal isolate recovered. The amplified products were separated using gel electrophoresis for the AP-PCR and a sequencer for the AFLP. Band patterns were then analyzed to create dendrograms. In each flock more strains of M. ovipneumoniae were detected when using AP-PCR compared to AFLP. Genomic fingerprints created from the AP-PCR technique yielded fewer bands than those from AFLP. Overall, it was confirmed that multiple strains of M. ovipneumoniae are involved in each flock infection.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6a27c9c60f92f913d8fafa0003750ae7 https://doi.org/10.1016/j.smallrumres.2007.01.013 Zobrazit plný text záznamu Full Text from ScienceDirect