The recognition of DNA cleavage sites by porcine splean topoisomeraseII topoisomerase II

Autor:	Hon-Ju Liu, Jin-Kai Juang, Hurng Wern Huang
Rok vydání:	1992
Předmět:	chemistry.chemical_classification Dna duplex biology Topoisomerase Energy analysis DNA sequencing chemistry.chemical_compound Enzyme chemistry Biochemistry Dna cleavage Correlation analysis Genetics biology.protein DNA
Zdroj:	Nucleic Acids Research. 20:467-473
ISSN:	1362-4962 0305-1048
Popis:	The cutting sites specificity of topoisomerase II from porcine spleen were determined by a modified Sanger's DNA sequencing method. The topoisomerase II prefers to cut DNA at the 3' side of A and leave 5' protruding end with two staggering bases. Through the free energy analysis for DNA duplex, we also found that the topoisomerase II seemed cut DNA preferably at energetically unstable regions. So it is concluded that the specific DNA cutting by porcine spleen topoisomerase II has two structural recognition factors: one is to localize around the energetically unstable region and another is to act at the 3' side of A base.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::6a24ee67bd4eee3beb6fc88ab3f4d294 https://doi.org/10.1093/nar/20.3.467 Zobrazit plný text záznamu