PTEN expression in human cumulus cells is associated with embryo development competence
| Autor: | Jianfeng Yao, Ming Li, Liqing Lin, Youzhu Li, Jianlong Zhuang, Yanfang Huang, Weilin Peng, Jianfeng Lian, Rongfu Huang, Xiaoyu Yang |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Zygote. 30:611-618 |
| ISSN: | 1469-8730 0967-1994 |
| Popis: | SummaryEmbryo quality determines the success of in vitro fertilization and embryo transfer (IVF-ET) treatment. Biomarkers for the evaluation of embryo quality have some limitations. Apoptosis in cumulus cells (CCs) is important for ovarian function. PTEN (phosphatase and tensin homolog) is a well known tumour suppressor gene that functions as a mediator of apoptosis and is crucial for mammalian reproduction. In the present study, we analyzed the expression level of PTEN in human CCs and aimed to investigate its association with embryo developmental competence in IVF treatment cycles. The PTEN mRNA level in CCs was measured using real-time fluorescence quantitative PCR. The association of the differential expression of PTEN with embryo quality was analyzed. Our data showed that PTEN mRNA levels were significantly decreased in CCs surrounding mature oocytes compared with immature oocytes. Similar changes were found in the analysis of fertilization and blastocyst formation. The speculation that the measurement of PTEN mRNA levels in human CCs would provide a useful tool for selecting oocytes with greater chances to implant into the uterus needs to be further verified through single-embryo transfer in the future. The proapoptotic mechanism of PTEN in human reproduction needs to be further studied. |
| Databáze: | OpenAIRE |
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