Immunological Study of Hydrophobic Polypeptides in Beer
| Autor: | E. N. C. Mills, Jørn Hejgaard, A. Onishi, J. M. Field, Michael R. A. Morgan, J. A. Kauffman, M. O. Proudlove |
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| Rok vydání: | 1998 |
| Předmět: |
Ammonium sulfate
Aqueous solution High molecular mass Chromatography biology Anticorps monoclonal medicine.drug_class food and beverages General Chemistry Monoclonal antibody Epitope Propanol chemistry.chemical_compound chemistry Biochemistry medicine biology.protein Antibody General Agricultural and Biological Sciences |
| Zdroj: | Journal of Agricultural and Food Chemistry. 46:4475-4483 |
| ISSN: | 1520-5118 0021-8561 |
| Popis: | A panel of 15 monoclonal antibodies (mAbs) has been raised to a fraction of lager beer protein precipitated with ammonium sulfate at 40% saturation. The antibodies have been characterized in terms of their binding to a set of Octyl-Sepharose fractions of lager beer foam with increasing hydrophobicity (groups 1-5) by enzyme-linked immunosorbent assay and immunoblotting methods. The epitopes recognized were present in all of the lager beer foam fractions to some degree, and many of the mAbs bound to both high molecular mass (MM) (>3000 Da) and low MM ( |
| Databáze: | OpenAIRE |
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