| Popis: |
Understanding the taxonomiy of the tropical genus Clusia (Fam. Clusiaceae, Ord. Theales) has been hampered by the difficulties inherent in studying tropical dioecious, succulent, arborescent, epi- or hemiepiphytic taxa. Species identification by morphological traits often requires the terminal inflorescences and/or the succulent capsular fruits. To allow species differentiation based exclusively on vegetative tissue, a frequent necessity during ecological field studies, a procedure has been developed for rapid isolation of genomic DNA from Clusia leaf tissue followed by DNA amplification fingerprinting with a set of single arbitrary oligomer primers (23–27 mers). Fingerprints obtained with independent DNA preparations from one individual as well as DNA preparations from several individuals of the same species were identical for the major amplification products, although minor bands were somewhat variable. Polymorphic fingerprints have been obtained with 3 different primers for 3 Clusia species (C. minor L., C. alata Pl. & Tr., C. multiflora H. B. K.), and the related Oedematopus obovatus Spruce ex. PL (Clusiaceae). The interspecific Randomly Amplified Polymorphic Markers (RAPDs) thus obtained allow a rapid identification of vegetative tissue samples collected in the field, and will assist in a revision of the controversial taxonomy of the genus Clusia. |
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