Preparation of silicon nitride biochips for reflectometric interference spectroscopic (RIfS) analysis of biological toxins and E. coli O157:H7 strain
| Autor: | Yasuo Seto, Tsuyoshi Iwasa, Takehiro Nagatsuka, Yoshihiro Nishida, Daiki Tanaka, Toshio Yoshida, Yuki Oba, Hirotaka Uzawa, Takayuki Ezaki, Ken-ichi Tayama |
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| Rok vydání: | 2017 |
| Předmět: |
Analytical chemistry
02 engineering and technology 010402 general chemistry medicine.disease_cause 01 natural sciences chemistry.chemical_compound Materials Chemistry medicine Electrical and Electronic Engineering Biochip Instrumentation Escherichia coli Chromatography biology Chemistry Toxin Metals and Alloys Substrate (chemistry) Lectin biochemical phenomena metabolism and nutrition 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Ricin Reflectometric interference spectroscopy Silicon nitride biology.protein 0210 nano-technology |
| Zdroj: | Sensors and Actuators B: Chemical. 246:937-942 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2017.02.136 |
| Popis: | The surface of a commercially available silicon nitride (SiN) substrate was modified with gold (Au) nanoparticle and then with either lactosyl derivative or monoclonal antibody. Each of the derived SiN chips carrying Au-lactose or Au-antibody was evaluated in the reflectometric interference spectroscopic (RIfS) analyses of Ricinus communis lectin (RCA 120 ) and toxin (RCA 60 ). The RIfS analysis using the Au-lactose/SiN chip enabled us to detect the plant lectin at concentrations less than 5–10 ng/mL as well as the toxin (ricin) sealed carefully in a test sample at concentration about 1 μg/mL. The RIfS analysis using the Au-antibody/SiN chip was useful for the facile and direct screening of enterohemorrhagic Escherichia coli (EHEC) O157:H7 strain in the presence of possible interfering materials. The overall detection was completed within 2.5 h-cultivation time without any purification process for the pathogenic bacterium. |
| Databáze: | OpenAIRE |
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