Determination of adhesion force between single cell pairs generated by activated GpIIb-IIIa receptors

Autor:	KL Sung, MM Frojmovic, TE O'Toole, C Zhu, MH Ginsberg, S Chien
Rok vydání:	1993
Předmět:	congenital hereditary and neonatal diseases and abnormalities hemic and lymphatic diseases Immunology hemic and immune systems Cell Biology Hematology Biochemistry circulatory and respiratory physiology
Zdroj:	Blood. 81:419-423
ISSN:	1528-0020 0006-4971
DOI:	10.1182/blood.v81.2.419.bloodjournal812419
Popis:	A biophysical approach was used to directly determine the avidity of the junction between two Chinese hamster ovary (CHO) cells bearing recombinant GpIIb-IIIa in the presence and absence of fibrinogen. Micromanipulation was used to induce conjugation of the cell pairs with or without activating the GpIIb-IIIa molecules with monoclonal antibody (MoAb) 62. Activation of GpIIb-IIIa caused an increase in the force required to separate the conjugates. The molecular bonding force between cells bearing activated GpIIb-IIIa and fibrinogen molecules was found to be 2.1 x 10(-7) dyne, which is 3.7 times higher than that between nonactivated GpIIb-IIIa and fibrinogen (5.7 x 10(-8) dyne). The results provide a quantitative assessment of the molecular bonding force between fibrinogen and the GpIIb-IIIa expressed on cell surface. The findings indicate that the activation of GpIIb-IIIa leads to an increase in the adhesive force in CHO cell aggregation by increasing the strength of the GpIIb-IIIa-fibrinogen bonds rather than the number of these bonds.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::69fb548b450692c2b86ae8be1e5b0343 https://doi.org/10.1182/blood.v81.2.419.bloodjournal812419 Zobrazit plný text záznamu