A gene expression profile for non-smoking and non-drinking patients with head and neck cancer
| Autor: | G. J. Hordijk, F. Farshadpour, Paul Roepman, Ron Koole, Pieter J. Slootweg |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Oncology
medicine.medical_specialty business.industry Microarray analysis techniques Head and neck cancer medicine.disease Head and neck squamous-cell carcinoma Gene expression profiling Oropharyngeal Neoplasm Otorhinolaryngology Internal medicine Gene expression Immunology Medicine DNA microarray business General Dentistry Gene |
| Zdroj: | Oral Diseases. 18:178-183 |
| ISSN: | 1354-523X |
| DOI: | 10.1111/j.1601-0825.2011.01861.x |
| Popis: | OBJECTIVE: A small subset of patients with head and neck squamous cell carcinoma are non-smoking and non-drinking and have distinct clinical characteristics. We aimed to identify a possible different genetic profile for these patients when compared with their smoking and drinking counterparts. MATERIALS AND METHODS: The gene expression data previously detected from primary tumors located in the oral cavity and oropharynx, using DNA microarray was analyzed for their differential expression between non-smoking and non-drinking patients (n = 15) and smoking and drinking patients (n = 89). Student's T-test (P < 0.05) and 10-fold cross-validation procedure (100 times repeated) were performed to determine differentially expressed genes. RESULTS: Non-smoking and non-drinking patients were older, mostly female and had oral cavity-localized tumors, whereas smoking and drinking patients were younger male patients with 81% oral cavity and 19% oropharynx tumors. A set of 49 differentially expressed genes were detected. Among others, seven genes related to interferon-gamma were upregulated and two genes linked to NFKB pathway were downregulated. CONCLUSIONS: Differentially expressed genes in non-smoking and non-drinking patients possibly indicate the presence of a different cellular response to carcinogenic events in these patients. Further studies are warranted to validate this gene set and explore possible therapeutic implications to improve prognosis for these patients. |
| Databáze: | OpenAIRE |
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