Proteomic Approach Analysis of Mammary Membrane Proteins Expression Profiles in Holstein Cows

Autor:	Yong Zhang, Sui-zhong Cao, Yong-xin Yang, Xing-xu Zhao
Rok vydání:	2009
Předmět:	Gel electrophoresis medicine.medical_specialty Mammary gland Biology Proteomics Endocrinology medicine.anatomical_structure Biochemistry Membrane protein Internal medicine Gene expression Proteome medicine Animal Science and Zoology Secretion Centrifugation Food Science
Zdroj:	Asian-Australasian Journal of Animal Sciences. 22:885-892
ISSN:	1976-5517 1011-2367
DOI:	10.5713/ajas.2009.80472
Popis:	To investigate host defense mechanisms for protecting the mammary gland from mastitis infection, the membrane fraction of mammary tissues from Holstein cows was purified by differential velocity centrifugation, and then the sodium dodecyl sulfate-polyacrylamid gel electrophoresis (SDS-PAGE) separated proteins were identified by ion trap mass spectrometer equipped with a Surveyor high performance liquid chromatography (HPLC) system. A total of 183 proteins were identified. Bioinformatics software was applied to analyse physicochemical characteristics of the identified proteins and to predict biochemical function. These data may provide valuable information to investigate the mechanisms of mammary gland milk secretion and infectious disease, and enable a clear identification of proteins and potential protein targets for therapies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::69f51878aa00e91171ceb780b50e5bed https://doi.org/10.5713/ajas.2009.80472 Zobrazit plný text záznamu