Thermal lens determination of cytochrome c and its NO complex

Autor: Andreyan N. Osipov, Mikhail A. Proskurnin, E. V. Proskurnina, Yu. A. Vladimirov, Anton V. Brusnichkin, Artem Marikutsa
Rok vydání: 2008
Předmět:
Zdroj: Moscow University Chemistry Bulletin. 63:338-342
ISSN: 1935-0260
0027-1314
DOI: 10.3103/s0027131408060072
Popis: Spectrophotometric and thermal lens measurements showed that cw laser beam (450–530 nm, up to 100 mW) does not affect the absorption band of cytochrome c. Therefore, thermal lensing is used for determining cytochrome c (III) (c min = 1 × 10−7 mol/L at λ = 488.0 nm; c min = 3 × 10−8 mol/L at λ = 514.5 nm) and its active form, cytochrome c (II) (c min = 1 × 10−8 mol/L at λ = 514.5 nm). The enhancement of the sensitivity of determination of these species as compared with conventional spectrophotometry is more than two orders of magnitude. The optimal conditions for the formation of the NO complex of cytochrome c for its photometric determination were selected: the molar ratio of dodecyl sulfate (a modifying agent) to cytochrome c is 1: 30 at a working wavelength of 560 nm. When exposed to laser radiation, the nitrosyl complex of cytochrome c dissociates to form cytochrome c (III). The decomposition of this complex can be monitored by thermal lensing (514.5 nm) down to a level of 1 × 10−7 mol/L.
Databáze: OpenAIRE
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