| Autor: |
Haixiang Ma, Leyuan Gu, Yuling Wang, Qing Xu, Weihui Shao, Qian Yu, Xiting Lian, Lu Liu, Jiaxuan Gu, Na Ji, Yuanli Zhang, Xiaoling Liu, Kazuki Nagayasu, Honghai Zhang |
| Rok vydání: |
2023 |
| DOI: |
10.21203/rs.3.rs-2665304/v1 |
| Popis: |
General anesthesia is widely used in various clinical practices due to its ability to cause loss of consciousness. However, the exact mechanism of anesthesia-induced unconsciousness remains unclear. It is generally thought that arousal-related brain nuclei are involved. 5-hydroxytryptamine (5-HT) is closely associated with sleep arousal. Here, we explore the role of the 5-HT system in anesthetic awakening through pharmacological interventions and optogenetic techniques. Our data showed that exogenous administration of 5-hydroxytryptophan (5-HTP) and optogenetic activation of 5-HT neurons in the dorsal raphe nucleus (DR) could significantly shorten the emergence time of sevoflurane anesthesia in mice, suggesting that regulation of the 5-HT system using both endogenous and exogenous approaches could mediate delayed emergence. In addition, we first discovered that the different 5-HT receptors located in the DR, known as 5-HT autoreceptors, are essential for the regulation of general anesthetic awakening, with 5-HT1A and 5-HT2A/C receptors playing a regulatory role. These results can provide a reliable theoretical basis as well as potential targets for clinical intervention to prevent delayed emergence and some postoperative risks. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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