Zinc deficiency in rats decreases thrombin-stimulated platelet aggregation by lowering protein kinase C activity secondary to impaired calcium uptake
| Autor: | Jinming Xia, Boyd L. O'Dell |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Nutrition and Dietetics Kinase Endocrinology Diabetes and Metabolism Clinical Biochemistry chemistry.chemical_element Zinc Calcium Biochemistry Adenosine diphosphate chemistry.chemical_compound Thrombin Endocrinology chemistry Internal medicine medicine Platelet Platelet activation Molecular Biology Protein kinase C medicine.drug |
| Zdroj: | The Journal of Nutritional Biochemistry. 6:661-666 |
| ISSN: | 0955-2863 |
| DOI: | 10.1016/0955-2863(95)00150-6 |
| Popis: | Aggregation of rat platelets, when stimulated by adenosine diphosphate (ADP) or fluoride, is impaired by zinc deficiency, and the defect is associated with a decreased uptake of external Ca2+. Zinc deficiency also impairs the aggregatory response of platelets to phorbol myristate acetate (PMA), an activator of protein kinase C, but low zinc status decreases the PMA response only when calcium is added to the external medium. The purpose of this study was to determine the role of protein kinase C in rat platelet function and its relationship to the zinc deficiency pathology observed in platelets stimulated by thrombin (THR). The percent of maximal aggregation and the concentration of cytosolic-free Ca2+ were measured in washed platelets stimulated by THR and PMA. For the protein kinase C experiments platelets were obtained from rats fed a grain-based diet, and for the thrombin experiments they were from rats fed purified diets. In the latter experiments, immature male rats were fed for 2 weeks a low zinc diet ( |
| Databáze: | OpenAIRE |
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