Transgenic improvement of photosynthetic property in tobacco using E12 .OMEGA. promoter system to express higher level of mouse carbonic anhydrase activity in cytoplasm

Autor:	Kenji Jinushi, Ryu Ohsugi, Ken Ishimaru, Ryuichi Ishii, Keiichiro Okabe, Tatsuro Hirose
Rok vydání:	1999
Předmět:	biology Transgene Carbon fixation Wild type food and beverages Plant Science Photosynthesis Biochemistry Cytoplasm Carbonic anhydrase Complementary DNA biology.protein Agronomy and Crop Science Chlorophyll fluorescence Biotechnology
Zdroj:	Plant Biotechnology. 16:353-359
ISSN:	1347-6114 1342-4580
DOI:	10.5511/plantbiotechnology.16.353
Popis:	Genetic challenge to express higher carbonic anhydrase (CA) activity in the cytoplasm of tobacco mesophyll cells was carried out by an improved promoter (El2 Ω) with a cDNA of partially modified mouse CA. In leaves of T1 progenies of transgenic tobacco (ffCA plant), the expressed mouse CA peptide was confirmed with 1, 5-3.2 times higher than the transgenic tobacco having foreign mouse CA controlled by simple CaMV 35S promoter (fCA plant). The ffCA plants manifested higher photosynthetic carbon assimilation (PCA) rate under ambient CO2 condition than wild type plants. The chlorophyll fluorescence measurement analysis revealed that the ffCA plant's improved PCA rate is related to the reduction of mesophyll CO2 transfer resistance (Rr) with little change in the stomatal resistance (Rs) or mesophyll CO2 fixation resistance (Rx). Moreover, ffCA plants showed a higher growth rate under high light condition.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::69e82ace75c5f6c7770509723585df40 https://doi.org/10.5511/plantbiotechnology.16.353 Zobrazit plný text záznamu