Physical map of a Plutella xylostella granulovirus genome

Autor:	Ei ichi Shimojo, Robert R. Granados, Yasumasa Ueno, Atsushi Kondo, Yoshifumi Hashimoto, Yoshitaka Sano, Tohru Hayakawa, Tsuguo Matsumoto, Minoru Miyasono, Kana Hayashi
Rok vydání:	2000
Předmět:	Genetics XhoI biology EcoRI biochemical phenomena metabolism and nutrition HindIII Molecular biology Genome Restriction enzyme Plasmid Granulovirus Insect Science biology.protein bacteria BamHI
Zdroj:	Applied Entomology and Zoology. 35:45-51
ISSN:	1347-605X 0003-6862
DOI:	10.1303/aez.2000.45
Popis:	A plasmid library of restriction DNA fragments of a Plutella xylostella granulovirus (PxGV) was constructed, which covered all 9 EcoRI and all 19 HindIII fragments, and 18 of 20 PstI fragments. Restriction endonuclease analysis and DNA-DNA hybridization analysis of the cloned viral DNA fragments in the library and the restricted viral DNA fragments were carried out for construction of a physical map of PxGV DNA for ApaI, BamHI, BglII, EcoRI, HindIII, KpnI, PstI, SacII, XbaI and XhoI. The size of PxGV genome was estimated to be 102.2 kb. Location of the left-most DNA fragment on the physical map that contained granulin gene was determined by hybridization using a DNA probe of granulin gene of Trichoplusia ni granulovirus. Four homologous regions interspersed on the genome were also determined by DNA-DNA hybridization analysis.
Databáze:	OpenAIRE
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