Betulinic Acid Inhibits LPS-Induced MMP-9 Expression by Suppressing NF-kB Activation in BV2 Microglial Cells
| Autor: | Jae-Won Lee, Yong-Joon Choi, Song-In Kim, Sue-Young Lee, Sang-Soo Kang, Nam-Ho Kim, Yong-Soo Kwon, Hee-Jae Lee, Wan-Joo Chun, Sung-Soo Kim |
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| Rok vydání: | 2011 |
| Předmět: |
Pharmacology
Microglia Lipopolysaccharide medicine.medical_treatment Stimulation Matrix metalloproteinase Biology Biochemistry Proinflammatory cytokine Cell biology chemistry.chemical_compound medicine.anatomical_structure Cytokine chemistry Betulinic acid Drug Discovery medicine Molecular Medicine Transcription factor |
| Zdroj: | Biomolecules and Therapeutics. 19:431-437 |
| ISSN: | 1976-9148 |
| DOI: | 10.4062/biomolther.2011.19.4.431 |
| Popis: | The objective of the present study was to evaluate anti-inflammatory effects of betulinic acid on lipopolysaccharide (LPS)-stimulated BV2 microglial cells. Pretreatment of betulinic acid significantly attenuated LPSinduced NO production and protein expression of iNOS. Betulinic acid also significantly suppressed LPS-induced release and expression of cytokines such as TNF-α and IL-1β. Furthermore, betulinic acid significantly suppressed LPSinduced MMP-9 expression, which has been suggested to play an important role in the migration of activated microglia. In order to understand the possible mechanism by which betulinic acid suppresses LPS-induced cytokine production and migration of microglia, the role of NF-kB, a major proinflammatory transcription factor, was examined. Betulinic acid significantly suppressed LPS-induced degradation of IkB, which retains NF-kB in the cytoplasm. Therefore, nuclear translocation of NF-kB upon LPS stimulation was significantly suppressed bybetulinic acid. Taken together, the present study for the first time demonstrates thatbetulinic acid possesses anti-inflammatory activity and inhibition of microglial migration through the suppression of nuclear translocation of NF-kB in BV2 microglial cells. |
| Databáze: | OpenAIRE |
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