High-resolution 3D reconstruction of human oocytes using FIB-SEM
| Autor: | Jakub Javůrek, Pavel Otevřel, Soňa Kloudová, Aleš Hampl, Zuzana Holubcová, Drahomíra Kyjovská, Martina Tatíčková, Zuzana Trebichalská |
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| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
030219 obstetrics & reproductive medicine 3D reconstruction High resolution Biology Oocyte law.invention Cell biology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure law Cytoplasm Organelle Ultrastructure medicine Volume reconstruction Electron microscope 030304 developmental biology |
| Popis: | The oocyte plays a pivotal role in the reproduction of our species. Nevertheless, its biology remains poorly understood. Electron microscopy is traditionally used to inspect the ultrastructure of female gametes. However, two-dimensional micrographs contain only fragmentary information about the spatial organization of the complex oocyte cytoplasm. Here, we employed the Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) to explore human oocyte intracellular morphology in three dimensions (3D). Volume reconstruction from high-resolution image stacks provided an unprecedented view of ooplasmic architecture. Organelle distribution patterns observed in 9 donor oocytes, representing 3 maturational stages, documented structural changes underlying the process by which the egg acquires developmental competence. 3D image segmentation was performed to extract information about distinct organelle populations. The quantitative analysis of the organelle abundance revealed that mitochondrion occupies ~ 4.26 % of the maturing oocyte cytoplasm. This proof-of-concept study demonstrates the potential of FIB-SEM imaging to study human oocyte morphology. |
| Databáze: | OpenAIRE |
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