Metal oxide semiconductor field-effect transistor (MOSFET)-based direct monitoring of p53 in spiked serum
| Autor: | Chang-Soo Lee, Yunkwang Oh, Young Hee Lee, Tran Thi Dung, Moonil Kim |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science General Chemical Engineering 010401 analytical chemistry Transistor Analytical chemistry 01 natural sciences 0104 chemical sciences law.invention Metal 03 medical and health sciences 030104 developmental biology Oxide semiconductor Ionic strength law visual_art MOSFET visual_art.visual_art_medium Direct monitoring Field-effect transistor Biosensor |
| Zdroj: | Journal of Industrial and Engineering Chemistry. 37:95-100 |
| ISSN: | 1226-086X |
| DOI: | 10.1016/j.jiec.2016.03.015 |
| Popis: | We describe the direct monitoring of p53 in a human serum sample by a metal oxide semiconductor field-effect transistor (MOSFET) biosensor. The biomolecular interaction between DNA and p53 protein was measured using a DNA-modified MOSFET under the condition of physiological ionic strength. Treated with p53 in serum, a MOSFET exhibited a comparable change in the drain current, while no alteration in response to serum spiked with DNA binding-defective p53 was observed, leading to a good agreement between theory and experiment. Our data suggest that a MOSFET device can be potentially used for the determination of p53 in serum samples on the basis of DNA–protein interaction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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