Embryo-to-embryo variability in RNAi knockdown efficiency of dKDM5/lid in Drosophila melanogaster

Autor:	Michael B. Eisen, Ashley R. Albright
Rok vydání:	2021
Předmět:	Small hairpin RNA Gene knockdown biology RNA interference Gene expression Embryo Drosophila melanogaster biology.organism_classification Cell biology
DOI:	10.1101/2021.04.29.442033
Popis:	We used the maternal-Gal4 shRNA system to knock down expression of dKDM5/lid in Drosophila melanogaster embryos, and analyzed the efficacy of the knockdown by qRT-PCR. Although average relative expression of lid was significantly lower in knockdown conditions compared to the driver-only control, we observed a wide and overlapping range of relative gene expression between individual control and knockdown embryos.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::69d65850a7543330eb45806bc71ab2fe https://doi.org/10.1101/2021.04.29.442033 Zobrazit plný text záznamu