| Autor: |
Edward G. Sedgwick, Philip D. Bragg, Cynthia Hou |
| Rok vydání: |
1984 |
| Předmět: |
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| Zdroj: |
Biochimica et Biophysica Acta (BBA) - Bioenergetics. 767:479-492 |
| ISSN: |
0005-2728 |
| DOI: |
10.1016/0005-2728(84)90046-x |
| Popis: |
The effects of carbonyl cyanide m-chlorophenylhydrazone (CCCP) and tri-n-butyltin chloride (Bu3SnCl) on proline transport, proton uptake and the transmembrane pH gradient in intact cells have been compared in a CCCP-resistant mutant strain Escherichia coli UV6, and its parent strain, AN180. CCCP and Bu3SnCl inhibited proline uptake in AN180 but the pH gradient was affected only by CCCP. Neither uncoupler affected the pH gradient in UV6 although inhibition of proline uptake occurred at high concentrations. CCCP caused efflux of accumulated proline in both strains but Bu3SnCl was ineffective. Bu3SnCl did not prevent the efflux of proline induced by CCCP, indicating that Bu3SnCl had not inactivated the transport carrier. In contrast with the parent strain, CCCP failed to reverse the oxidation-dependent inhibition of the phosphotransferase system in UV6 even at concentrations causing inhibition of proline uptake. The phosphorylation potential of UV6 with succinate as substrate was lower than in AN180. This was associated with a 10-fold higher concentration of phosphate in succinate-grown UV6 than in AN180. These results suggest that CCCP and Bu3SnCl have different sites of action on the membrane energization system of intact cells of E. coli. A possible explanation of the differences between AN180 and UV6 is that the energization system is altered in the CCCP-resistant mutant. Both uncouplers stimulated the uptake of protons by intact cells to the same extent in UV6 as in AN180. In UV6, and in AN180 with Bu3SnCl, this was not accompanied by effects on the transmembrane pH gradient. The extent of proton uptake appeared to be related to the level of the highly anionic membrane-associated oligosaccharides in the periplasmic space. It is proposed the outer membrane acts as a partial barrier to protons and that the uncouplers can equilibrate protons between the extracellular medium and the periplasmic space in intact cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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