Efficient Resolution of cis-(±)-Dimethyl 1-Acetylpiperidine-2,3-dicarboxylate by Covalently Immobilized Mutant Candida antarctica Lipase B in Batch and Semicontinuous Modes

Autor:	Zhiqiang Liu, Jia-Mei Qi, Jiang-Wei Shen, Yu-Guo Zheng, Zhang Xiaojian
Rok vydání:	2019
Předmět:	Packed bed biology Chemistry Bioconversion Organic Chemistry Continuous stirred-tank reactor biology.organism_classification chemistry.chemical_compound Biocatalysis Covalent bond Thermal stability Candida antarctica Glutaraldehyde Physical and Theoretical Chemistry Nuclear chemistry
Zdroj:	Organic Process Research & Development. 23:1017-1025
ISSN:	1520-586X 1083-6160
DOI:	10.1021/acs.oprd.9b00066
Popis:	Candida antarctica lipase B (CALB) is a robust biocatalyst for production of chemicals. In this study, an engineered lipase B (CALB-I189K) was covalently immobilized onto a glutaraldehyde activated amino resin (ESR-3) and used for the preparation of chiral moxifloxacin precursor though enzymatic resolution of cis-(±)-dimethyl 1-acetylpiperidine-2,3-dicarboxylate (cis-(±)-1). The immobilization conditions were optimized, and the immobilized CALB-I189K exhibited improved thermal stability, with a half-life of 247.5 h at 30 °C. It enables the resolution of 100 g L–1 cis-(±)-1 for 50 cycles in a stirred tank reactor, with an average productivity of 49.6 g L–1 h–1. The resolution reaction was also performed in a recirculating packed bed reactor (RPBR) in a semicontinuous mode, and the average productivity reached 59.4 g L–1 h–1 under the optimal operating condition. Moreover, the RPBR bioconversion system has high operational stability and conserved over 85.7% of the initial conversion capacity after 50 cycles...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::69c646428ea3b8add8fe141979921081 https://doi.org/10.1021/acs.oprd.9b00066 Zobrazit plný text záznamu