Characterization of acylcarnitines as their isobutyl ester derivatives using fast atom bombardment mass spectrometry and constant neutral loss scan

Autor: R. A. Chalmers, B. M. Tracey, K. N. Cheng, J. Rosankiewicz
Rok vydání: 1989
Předmět:
Zdroj: Biological Mass Spectrometry. 18:668-672
ISSN: 1096-9888
0887-6134
DOI: 10.1002/bms.1200180906
Popis: A simple method is described for the characterization of acylcarnitines by fast atom bombardment mass spectrometry in combination with constant neutral loss (CNL) scan. Acylcarnitines in urine are extracted using cation-exchange chromatography and derivatized to their isobutyl esters. The fragmentation patterns of these compounds are studied by various linked-scan methods including B/E, B2/E and B/E√(1 – E) scans. Acylcarnitine isobutyl esters are found to lose two specific neutral fragments simultaneously so that a CNL scan of the combined mass produces a much enhanced spectrum without most of the background peaks. An example is given in which the CNL scan is used to diagnose a patient with medium-chain acyl CoA dehydrogenase deficiency. The use of linked-scan techniques to monitor a specific metastable ion transition can be exploited for characterizing structurally related compounds and therefore the technique reported in this paper may have much wider applications to mixture analysis.
Databáze: OpenAIRE
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